Difference between revisions of "Part:BBa K3739060"

 
 
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pDawn promoter system can be activated by blue light.
 
pDawn promoter system can be activated by blue light.
  
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===Usage and Biology===
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===Biology and Usage===
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pDawn is a kind of regulation system that has been illustrated effectively in ''Vibrio natriegens'' which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site in its downstream.
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Latest revision as of 21:07, 21 October 2021


pDawn (LacIq-yf1-fixJ-pFixK2-cI-pR)

pDawn promoter system can be activated by blue light.


Biology and Usage

pDawn is a kind of regulation system that has been illustrated effectively in Vibrio natriegens which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site in its downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525