Difference between revisions of "Part:BBa K3806011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In part BBa_K3806011 the BBa_K3806008 sequence is linked downstream of a T7 promoter for its transcription by the T7 bacteriophage RNA polymerase. The sequence can be amplified by primers BBa_K3806017 and BBa_K3806020. | + | In part BBa_K3806011, the BBa_K3806008 sequence is linked downstream of a T7 promoter for its transcription by the T7 bacteriophage RNA polymerase. The sequence can be amplified by primers BBa_K3806017 and BBa_K3806020. |
===Source=== | ===Source=== | ||
Latest revision as of 20:58, 21 October 2021
Theophylline-binding aptazyme with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In part BBa_K3806011, the BBa_K3806008 sequence is linked downstream of a T7 promoter for its transcription by the T7 bacteriophage RNA polymerase. The sequence can be amplified by primers BBa_K3806017 and BBa_K3806020.
Source
The sequence of the Theophylline aptazyme was retrieved from the manuscript by Townshed et al. [1].
References
[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. and Smolke, C. (2021). A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nat Commun, 12, 1437.