Difference between revisions of "Part:BBa K4073000"
 
(6 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K4073000 short</partinfo>
 
<partinfo>BBa_K4073000 short</partinfo>
  
−
This composite part consists of a CrGDPH3 salt-inducible promoter, a medium-strength RBS, the AtHKT1 gene, and a terminator. The regulation of the expression of the AtHKT1 gene is the function of this part. The CrGPDH3 salt-inducible promoter was used for the overexpression of proteins in a transgene in microalga Chlamydia reinhardtii.  We then decided to utilize this promoter because of its high NaCl response elements. The RBS initiates translation of the HKT1 gene, which is essential for its protein synthesis. Finally, we used a composite terminator which signals the end of transcription in our part. This is essential, as if we did not utilize a terminator, the AtHKT1 gene could potentially be over-expressed, leading to decreased salt concentrations which could inhibit plant growth (salt is an essential nutrient used in plant growth).  
+
This composite part consists of a CrGDPH3 salt-inducible promoter, a medium-strength RBS, the AtHKT1 gene, and a terminator. The regulation of the expression of the AtHKT1 gene is the function of this part. The CrGPDH3 salt-inducible promoter was used for the over-expression of proteins in a transgene in microalga Chlamydia reinhardtii.  We then decided to utilize this promoter because of its high NaCl response elements. The RBS initiates translation of the HKT1 gene, which is essential for its protein synthesis. Finally, we used a composite terminator which signals the end of transcription in our part. This is essential, as if we did not utilize a terminator, the AtHKT1 gene could potentially be over-expressed, leading to decreased salt concentrations which could inhibit plant growth (salt is an essential nutrient used in plant growth).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 10: Line 10:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4073000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4073000 SequenceAndFeatures</partinfo>
−
 
+
https://static.igem.org/mediawiki/parts/a/ab/T--WVHS_SanDiego_CA--weakRBSnew.png
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K4073001 parameters</partinfo>
+
<partinfo>BBa_K4073000 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 20:18, 21 October 2021

BBa_K4073000 regulates salt tolerance in plants.

This composite part consists of a CrGDPH3 salt-inducible promoter, a medium-strength RBS, the AtHKT1 gene, and a terminator. The regulation of the expression of the AtHKT1 gene is the function of this part. The CrGPDH3 salt-inducible promoter was used for the over-expression of proteins in a transgene in microalga Chlamydia reinhardtii. We then decided to utilize this promoter because of its high NaCl response elements. The RBS initiates translation of the HKT1 gene, which is essential for its protein synthesis. Finally, we used a composite terminator which signals the end of transcription in our part. This is essential, as if we did not utilize a terminator, the AtHKT1 gene could potentially be over-expressed, leading to decreased salt concentrations which could inhibit plant growth (salt is an essential nutrient used in plant growth).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1912
    Illegal BsaI.rc site found at 225

T--WVHS_SanDiego_CA--weakRBSnew.png