Difference between revisions of "Part:BBa K3739027"

Latest revision as of 19:57, 21 October 2021

LamB1-LC1KR2-LamB2-his

We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use BBa_K3739057 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739027 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 612
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 760

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3739027 short</partinfo>
-We anchor LCI-KR2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use <partinfo>BBa_K3739057</partinfo> to construct the expression system and anchor LCI-KR2 on the surface of VnDX.
+We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use <partinfo>BBa_K3739057</partinfo> to construct the expression system and anchor LCIKR-2 on the surface of VnDX.
 ===Biology===
-LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCI-KR2 is fused with LamB used as a sandwich-anchoring motif so that LCI-KR2 can be displayed on the surface of VnDX.
+LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.
+===Usage===
+Here, we use BBa_3739027 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.
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