Difference between revisions of "Part:BBa K3739042"
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===Usage=== | ===Usage=== | ||
By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''BL21(DE3)'' was constructed. The constructed plasmid was transformed into ''BL21(DE3)'' to verify its expression. | By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''BL21(DE3)'' was constructed. The constructed plasmid was transformed into ''BL21(DE3)'' to verify its expression. | ||
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Latest revision as of 19:52, 21 October 2021
J23100-B0030-AKTA-his-Mfp5-B0010
It is a kind of mucin from mussels, including AKTA expression tags and 6xHis affinity tags which can let the mucin purified by tags.
Biology
Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It's side chain contains a lot of dopamine and it is the main factor of adhesion.
Usage
By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The constructed plasmid was transformed into BL21(DE3) to verify its expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]