Difference between revisions of "Part:BBa K3739024"

Revision as of 19:51, 21 October 2021

LamB-LC1KR2-GFP

We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene. We use BBa_K3739051 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused at C terminal with LamB so that LCIKR-2 can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739024 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.

Fig. 1. Gene circuit and agarose gel electrophoresis. (A) Gene circuit of LamB-LCIKR-2-GFP (BBa_K3739024). (B)Target bands of LamB-LCIKR-2-GFP (black arrow, 2300 bp).

Sequence and Features