Difference between revisions of "Part:BBa I742107"

 
(Usage and Biology)
 
(2 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I742107 short</partinfo>
 
<partinfo>BBa_I742107 short</partinfo>
Line 5: Line 4:
 
COMT coding sequence
 
COMT coding sequence
  
Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
+
Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring, converting caffeic acid to ferulic acid) from ''Medicago sativa'' (alfalfa). This gene was synthesised by Geneart.
  
  
<!-- Add more about the biology of this part here
+
IISER_BHOPAL 2021
 
===Usage and Biology===
 
===Usage and Biology===
 +
It has been found that strong downregulation of COMT in transgenic alfalfa plants decrease the lignin amount and a reduction in the number of units of guaiacyl (G) lignin with a total loss of syringyl (S) units in monomeric and dimeric lignin degradation products.[1]
 +
 +
<html>
 +
  <body>
 +
  <img src="https://2021.igem.org/wiki/images/thumb/4/41/T--IISER_Bhopal--Project--Contribution--image2.png/1200px-T--IISER_Bhopal--Project--Contribution--image2.png" style="width:100%">
 +
</body>
 +
</html>
  
<!-- -->
+
===References===
 +
1.Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell, 13(1), 73.
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_I742107 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_I742107 SequenceAndFeatures</partinfo>

Latest revision as of 19:44, 21 October 2021

COMT

COMT coding sequence

Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring, converting caffeic acid to ferulic acid) from Medicago sativa (alfalfa). This gene was synthesised by Geneart.


IISER_BHOPAL 2021

Usage and Biology

It has been found that strong downregulation of COMT in transgenic alfalfa plants decrease the lignin amount and a reduction in the number of units of guaiacyl (G) lignin with a total loss of syringyl (S) units in monomeric and dimeric lignin degradation products.[1]

References

1.Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell, 13(1), 73. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577