Difference between revisions of "Part:BBa I742107"
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COMT coding sequence | COMT coding sequence | ||
− | Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa) | + | Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring, converting caffeic acid to ferulic acid) from ''Medicago sativa'' (alfalfa). This gene was synthesised by Geneart. |
− | + | IISER_BHOPAL 2021 | |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | It has been found that strong downregulation of COMT in transgenic alfalfa plants decrease the lignin amount and a reduction in the number of units of guaiacyl (G) lignin with a total loss of syringyl (S) units in monomeric and dimeric lignin degradation products.[1] | ||
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+ | <html> | ||
+ | <body> | ||
+ | <img src="https://2021.igem.org/wiki/images/thumb/4/41/T--IISER_Bhopal--Project--Contribution--image2.png/1200px-T--IISER_Bhopal--Project--Contribution--image2.png" style="width:100%"> | ||
+ | </body> | ||
+ | </html> | ||
− | + | ===References=== | |
+ | 1.Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell, 13(1), 73. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_I742107 SequenceAndFeatures</partinfo> | <partinfo>BBa_I742107 SequenceAndFeatures</partinfo> |
Latest revision as of 19:44, 21 October 2021
COMT
COMT coding sequence
Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring, converting caffeic acid to ferulic acid) from Medicago sativa (alfalfa). This gene was synthesised by Geneart.
IISER_BHOPAL 2021
Usage and Biology
It has been found that strong downregulation of COMT in transgenic alfalfa plants decrease the lignin amount and a reduction in the number of units of guaiacyl (G) lignin with a total loss of syringyl (S) units in monomeric and dimeric lignin degradation products.[1]
References
1.Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell, 13(1), 73. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 577