Difference between revisions of "Part:BBa K3189004"
| Line 4: | Line 4: | ||
TetR binds to the tetracycline operator TetO to inhibit transcription. This effect can be relieved with the addition of tetracycline | TetR binds to the tetracycline operator TetO to inhibit transcription. This effect can be relieved with the addition of tetracycline | ||
| + | |||
| + | ==<strong>OUC-China 2021’s characterization</strong>== | ||
| + | ===Design=== | ||
| + | We use our special reporter gene 3WJDB(BBa_K3777000).When tet was absent, TetR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene. | ||
| + | |||
| + | ===Result=== | ||
| + | https://static.igem.org/mediawiki/parts/1/12/106-tetr%E2%91%A1.PNG | ||
| + | |||
| + | The figure shows that our cuircuit can recognize different concentrations of tetracycline, and the fluorescence value was positively correlated with tetracycline concentration which proves that our part can Detect the concentration of tetracycline,and our reporter gene 3WJDB is surprisingly sensitive. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 19:10, 21 October 2021
Tetracycline repressor TetR
TetR binds to the tetracycline operator TetO to inhibit transcription. This effect can be relieved with the addition of tetracycline
OUC-China 2021’s characterization
Design
We use our special reporter gene 3WJDB(BBa_K3777000).When tet was absent, TetR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene.
Result
The figure shows that our cuircuit can recognize different concentrations of tetracycline, and the fluorescence value was positively correlated with tetracycline concentration which proves that our part can Detect the concentration of tetracycline,and our reporter gene 3WJDB is surprisingly sensitive.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]