Difference between revisions of "Part:BBa K4080010:Design"
(One intermediate revision by the same user not shown) | |||
Line 13: | Line 13: | ||
<li> | <li> | ||
The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX | The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX | ||
+ | </li> | ||
+ | <li> | ||
+ | Addition of 6xHis-tag after amilCP | ||
</li> | </li> | ||
<li> | <li> | ||
Line 22: | Line 25: | ||
All of the parts are from the previous iGEM team that registered in the parts registry. | All of the parts are from the previous iGEM team that registered in the parts registry. | ||
− | |||
− |
Latest revision as of 19:01, 21 October 2021
amilCP-eCPX fusion with T7 expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- The system works under the T7 expression system, each part must be compatible and build strong-good relations
- The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
- Addition of 6xHis-tag after amilCP
- Linkers placed between parts : GGG or GSG
Source
All of the parts are from the previous iGEM team that registered in the parts registry.