Difference between revisions of "Part:BBa K3885352"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | 1.This plasmid has | + | 1. This plasmid has ssrA tag. Compare with P28-tetO-deGFP, we use it to investigate the effect of degradation ssrA tag on deGFP fluorescent protein.<br> |
− | 2.It can be used in other experiments which hope to express fluorescence with ssrA. | + | 2. It can be used in other experiments which hope to express fluorescence with ssrA. |
===Characterization=== | ===Characterization=== | ||
− | |||
<html> | <html> | ||
− | <div style=" | + | <style> |
− | <img src="https://static.igem.org/mediawiki/parts/1/1d/352_quxian.png" width= | + | .flex{ |
− | + | display: flex; | |
+ | align-items: center; | ||
+ | justify-content: space-evenly; | ||
+ | } | ||
+ | </style> | ||
+ | <div class="flex" style="margin: 0 auto; width: 35%;background-color: #f9f9f9;border: 1px solid #000;"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/1/1d/352_quxian.png" width=95%;style="display: block;margin:10px;10px;auto;"/> | ||
</div> | </div> | ||
− | </html><br> | + | </html> |
+ | This part provides ssrA tag. Compared with P28-tetO-deGFP, we can prove that degradation label will decrease the expression of fluorescent protein.<br> | ||
The result shows that the element which has no ssrA can present more fluorescence intensity. | The result shows that the element which has no ssrA can present more fluorescence intensity. | ||
Latest revision as of 18:48, 21 October 2021
P28-tetO-deGFP-ssrA
Under the P28 promoter, the Tet operator tetO is expressed along with the production of green fluorescent protein.Under the degradation of ssrA, the tet operator tetO is progressively degraded.
Usage and Biology
1. This plasmid has ssrA tag. Compare with P28-tetO-deGFP, we use it to investigate the effect of degradation ssrA tag on deGFP fluorescent protein.
2. It can be used in other experiments which hope to express fluorescence with ssrA.
Characterization
![](https://static.igem.org/mediawiki/parts/1/1d/352_quxian.png)
The result shows that the element which has no ssrA can present more fluorescence intensity.
Design Page
We want to know if the network would exhibit a pulse-like signal in TXTL when protein degradation machinery was added.
Usage and Biology
Melissa K. Takahashi et al. Characterizing and prototyping genetic networks with cell-free transcription–translation reactions[J]. Methods, 2015, 86 : 60-72.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]