Difference between revisions of "Part:BBa K3885352"

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===Usage and Biology===
 
===Usage and Biology===
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1.This plasmid has label ssrA . Compare with P28-tetO-deGFP, we use it to investigate the effect of degradation label ssrA on deGFP fluorescent protein.
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1. This plasmid has ssrA tag. Compare with P28-tetO-deGFP, we use it to investigate the effect of degradation ssrA tag on deGFP fluorescent protein.<br>
−
2.It can be used in other experiments which hope to express fluorescence with ssrA.
+
2. It can be used in other experiments which hope to express fluorescence with ssrA.
  
 
===Characterization===
 
===Characterization===
−
This element provides label ssrA . Compared with P28-tetO-deGFP, we can prove that degradation label will decrease the expression of fluorescent protein.
 
 
<html>
 
<html>
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<div style="border: 1px solid #000;margin: 0 auto;">
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<style>
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<img src="https://static.igem.org/mediawiki/parts/1/1d/352_quxian.png" width="300"/>
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.flex{
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<p>ahshkashdhak</p>
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    display: flex;
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    align-items: center;
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  justify-content: space-evenly;
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}
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</style>
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<div class="flex" style="margin: 0 auto; width: 35%;background-color: #f9f9f9;border: 1px solid #000;">
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<img src="https://static.igem.org/mediawiki/parts/1/1d/352_quxian.png" width=95%;style="display: block;margin:10px;10px;auto;"/>
 
</div>
 
</div>
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</html><br>
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</html>
 +
This part provides ssrA tag. Compared with P28-tetO-deGFP, we can prove that degradation label will decrease the expression of fluorescent protein.<br>
 
The result shows that the element which has no ssrA can present more fluorescence intensity.
 
The result shows that the element which has no ssrA can present more fluorescence intensity.
  

Latest revision as of 18:48, 21 October 2021


P28-tetO-deGFP-ssrA

Under the P28 promoter, the Tet operator tetO is expressed along with the production of green fluorescent protein.Under the degradation of ssrA, the tet operator tetO is progressively degraded.

Usage and Biology

1. This plasmid has ssrA tag. Compare with P28-tetO-deGFP, we use it to investigate the effect of degradation ssrA tag on deGFP fluorescent protein.
2. It can be used in other experiments which hope to express fluorescence with ssrA.

Characterization

This part provides ssrA tag. Compared with P28-tetO-deGFP, we can prove that degradation label will decrease the expression of fluorescent protein.
The result shows that the element which has no ssrA can present more fluorescence intensity.

Design Page

We want to know if the network would exhibit a pulse-like signal in TXTL when protein degradation machinery was added.

Usage and Biology

Melissa K. Takahashi et al. Characterizing and prototyping genetic networks with cell-free transcription–translation reactions[J]. Methods, 2015, 86 : 60-72.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]