Difference between revisions of "Part:BBa K3885111"

 
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<partinfo>BBa_K3885111 short</partinfo>
 
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It is a RBS that can continuously express the gene in E.coli, and with P70 promoter, it is as strong as the T7 promoter .
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UTR(Untranslated region (UTR) is the untranslated part at the 5' or 3' end of mature mRNA molecule, which generally plays an important role in mRNA transport, stability and translation regulation. The UTR1 sequence includes the T7 gene 10 leader RNA, which contains a strong ribosome binding site.  
  
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===Usage and Biology===
 
===Usage and Biology===
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<img src="https://static.igem.org/mediawiki/parts/d/d7/Utr12.png" width=95% style="display: block;margin: 10px auto;"/>
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<p style="text-align: center;">Figure 1: The sequence of UTR1. </p>
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In our experiment, we enhanced the expression of σ28 by adding UTR1 gene fragment before σ28 gene on the constructed plasmid. This part acts as a strong promoter. It can be combined with other genes to increase its expression.
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<p style="text-align: center;"> Figure 2. Comparing expression in TXTL reactions to the model. Maximum rate of deGFP synthesis expressed from varying concentrations of P70a-UTR1-deGFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3885311">(BBa_K3885311)</a>,  P70b-UTR1-deGFP  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3885321">(BBa_K3885321)</a>, P70c-UTR1-deGFP  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3885331">(BBa_K3885331)</a></p>
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Latest revision as of 18:30, 21 October 2021


UTR1

UTR(Untranslated region (UTR) is the untranslated part at the 5' or 3' end of mature mRNA molecule, which generally plays an important role in mRNA transport, stability and translation regulation. The UTR1 sequence includes the T7 gene 10 leader RNA, which contains a strong ribosome binding site.

Usage and Biology

Figure 1: The sequence of UTR1.


In our experiment, we enhanced the expression of σ28 by adding UTR1 gene fragment before σ28 gene on the constructed plasmid. This part acts as a strong promoter. It can be combined with other genes to increase its expression.

Figure 2. Comparing expression in TXTL reactions to the model. Maximum rate of deGFP synthesis expressed from varying concentrations of P70a-UTR1-deGFP (BBa_K3885311), P70b-UTR1-deGFP (BBa_K3885321), P70c-UTR1-deGFP (BBa_K3885331)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]