Difference between revisions of "Part:BBa K3739050"
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1. | After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1. | ||
| − | ::'''Fig. 1.''' Gene circuit | + | ::'''Fig. 1.''' Gene circuit and agarose gel electrophoresis. (A) Gene circuit of LamB-GFP (BBa_K3739050). (B) Target bands of LCIKR-2-GFP (black arrow, 1200 bp). |
Revision as of 18:21, 21 October 2021
J23100-B0030-LC1KR-2-GFP-B0010
LCIKR-2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. We use BBa_K3739050 to verify its capability by purification the protein.
Biology
LCIKR-2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly.. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with LCIKR-2 so that the expression of LCIKR-2 will be reported.
Characterization
1. Identification
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.
- Fig. 1. Gene circuit and agarose gel electrophoresis. (A) Gene circuit of LamB-GFP (BBa_K3739050). (B) Target bands of LCIKR-2-GFP (black arrow, 1200 bp).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 263