Difference between revisions of "Part:BBa K3739050"

Revision as of 18:15, 21 October 2021

J23100-B0030-LC1KR-2-GFP-B0010

LCI-KR2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. We use BBa_K3739050 to verify its capability by purification the protein.

Biology

LCIKR-2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly.. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with LCIKR-2 so that the expression of LCIKR-2 will be reported.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.

Fig. 1. Gene circuit involved in protein expression and AGE analysis of string a, Gene circuit was constructed by BBa_K3739050 to express LCIKR-2-GFP. b, Target bands of LCIKR-2-GFP (black arrow, 1200 bp).

Sequence and Features