Difference between revisions of "Part:BBa K3771077"

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	<br>CSAD was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC). <br>		<br>CSAD was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC). <br>
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−	<img src="https://2021.igem.org/wiki/images/a/aa/T--NCKU_Tainan--3.png" style="width:35%;">	+
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−	<p>Fig. 2 Taurine production of Ptrc-cdo1 +PlacI-csad +Ptrc-cs in different growth mediums.</p>	+
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−	<img src="https://2021.igem.org/wiki/images/6/6b/T--NCKU_Tainan--invivo1.png" style="width:35%;">	+
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−	<p>Fig. 3 Taurine production of Ptrc-cdo1+Ptrc-csad in different growth mediums.</p>	+
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−	<img src="https://2021.igem.org/wiki/images/f/f1/T--NCKU_Tainan--DkO.png" style="width:35%;">	+
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−	<p>Fig. 4 Taurine production (extracellular) of CDO1 and CSAD in <i>∆tauD</i>.</p>	+
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−	<img src="https://2021.igem.org/wiki/images/2/24/T--NCKU_Tainan--tauD2.png" style="width:35%;">	+
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−	<p>Fig. 5 Taurine production (extracellular) of CDO1 and CSAD in DkO.</p>	+
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−	<img src="https://2021.igem.org/wiki/images/d/dd/T--NCKU_Tainan--in.png" style="width:35%;">	+
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−	<p>Fig. 6 Taurine production (intracellular and extracellular) of CDO1 and CSAD in DkO and top10.</p>	+
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−	<img src="https://2021.igem.org/wiki/images/2/22/T--NCKU_Tainan--invivo4.png" style="width:35%;">	+
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−	<p>Fig. 7  Taurine production (extracellular) of CDO1 and CSAD with expression of CSAD regulated by soxS promoter in DkO.</p>	+
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Revision as of 18:14, 21 October 2021

Ptrc-CSAD

Description

P_trc-csad is a composite part consisting of the trc promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

trc promoter constitutively facilitates the expression of CSAD enzyme.

Usage

CSAD was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Characterization

Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)

Fig. 2 Confirmation of pSUI-Ptrc-CSAD by digestion. M: Marker; Lane 1~3: Different colonies of pSUI-Ptrc-CSAD (3674 bp)

Fig. 3 Transformation/CSAD in DH5α

Fig. 4 Confirmation of protein expression of CSAD. M: Marker; Lane1: whole cell of CSAD in DH5α; Lane2: soluble protein of CSAD in DH5α (~22 kDa)

References
Sequence and Features