Difference between revisions of "Part:BBa K4055517"
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This part is used to express Mfp3s-pep protein.  Mfp3s-pep （Mussel foot protein peptide）is the protein we selected from the papers of Yi Yu , Yi Cao , Chao Zhong and so on.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of HA.
 
This part is used to express Mfp3s-pep protein.  Mfp3s-pep （Mussel foot protein peptide）is the protein we selected from the papers of Yi Yu , Yi Cao , Chao Zhong and so on.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of HA.
  
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===Experiment===
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[[File:T--Yucai_SZ--1.png|500px|thumb|center|Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there are biofilms that are expressed]]
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<br><p>
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After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0).</p><br>
  
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[[File:T--Yucai_SZ--2.png|500px|thumb|center|SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm]]
  
 
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===Experiment===
 
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[[File:T--Yucai_SZ--1.png|500px|thumb|center|Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there are biofilms that are expressed]]
 
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<br><p>
 
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After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0).</p><br>
 
  
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[[File:T--Yucai_SZ--2.png|500px|thumb|center|SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm]]
 
 
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=References=
 
=References=
 
Living materials fabricated via gradient mineralization of light-inducible biofilms (DOI:10.1038/s41589-020-00697-z)
 
Living materials fabricated via gradient mineralization of light-inducible biofilms (DOI:10.1038/s41589-020-00697-z)

Revision as of 18:10, 21 October 2021


Mfp3S-pep adhesive protein

This part is used to express Mfp3s-pep protein. Mfp3s-pep （Mussel foot protein peptide）is the protein we selected from the papers of Yi Yu , Yi Cao , Chao Zhong and so on.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of HA.

Experiment

Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there are biofilms that are expressed

After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0).


SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




References

Living materials fabricated via gradient mineralization of light-inducible biofilms (DOI:10.1038/s41589-020-00697-z)