Difference between revisions of "Part:BBa K3838345"
Line 3: Line 3:
 
<partinfo>BBa_K3838345 short</partinfo>
 
<partinfo>BBa_K3838345 short</partinfo>
  
−
The overproduction of Superoxide dismutase (SOD) and reactive oxygen species (ROS) is considered to be one of the pathogenesis of IBD. The use of SOD can significantly reduce the peroxidation reaction in the inflamed colon. Studies have shown that the decreased expression of Cu/Zn-SOD has been observed in IBD patients. SOD can not be directly treated, so carrier transport is needed. Therefore, we selected for heteroexpression of superoxide dismutase (SOD) gene from Lactobacillus casei Lc18. In a team project, SOD related parts are composed as follows: J23100 promoter +RBS+SOD+ HIS tag +T7 terminator
+
The overproduction of Superoxide dismutase (SOD) and reactive oxygen species (ROS) is considered to be one of the pathogenesis of IBD. The use of SOD can significantly reduce the peroxidation reaction in the inflamed colon. Studies have shown that the decreased expression of Cu/Zn-SOD has been observed in IBD patients. SOD can not be directly treated, so carrier transport is needed. Therefore, we selected for heteroexpression of superoxide dismutase (SOD) gene from Lactobacillus casei Lc18.
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part hereST
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 17:58, 21 October 2021


YH-SOD

The overproduction of Superoxide dismutase (SOD) and reactive oxygen species (ROS) is considered to be one of the pathogenesis of IBD. The use of SOD can significantly reduce the peroxidation reaction in the inflamed colon. Studies have shown that the decreased expression of Cu/Zn-SOD has been observed in IBD patients. SOD can not be directly treated, so carrier transport is needed. Therefore, we selected for heteroexpression of superoxide dismutase (SOD) gene from Lactobacillus casei Lc18.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 534
    Illegal NheI site found at 147
    Illegal NheI site found at 170
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 534
    Illegal BglII site found at 577
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 534
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]