Difference between revisions of "Part:BBa K3729000"
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Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl-CoA-dehydrogenase from the genome of Faecalibacterium prausnitzii. | Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl-CoA-dehydrogenase from the genome of Faecalibacterium prausnitzii. | ||
| − | < | + | <b>Construct design:</b>We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT. | We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT. | ||
Revision as of 17:38, 21 October 2021
BCD 1: (Acyl-coa Dehydrogenase)
Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl-CoA-dehydrogenase from the genome of Faecalibacterium prausnitzii.
Construct design:We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT.
Sequence and Features
We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT.