Difference between revisions of "Part:BBa K3805999"
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We designed the sgRNA (19 bp) that can bind to the T7 promoter, which can guide the dCas9 to bind to the T7 promoter and inhibit the function of the promoter. | We designed the sgRNA (19 bp) that can bind to the T7 promoter, which can guide the dCas9 to bind to the T7 promoter and inhibit the function of the promoter. | ||
| − | == | + | ===How does it work?=== |
| − | Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination | + | Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination has the effect of inhibition of targeted gene transcription. This process can silence gene expression in cells effectively. |
[[File:DCas9.jpeg|500px|center]] | [[File:DCas9.jpeg|500px|center]] | ||
Latest revision as of 17:28, 21 October 2021
sgRNA bind to T7 promoter
We designed the sgRNA (19 bp) that can bind to the T7 promoter, which can guide the dCas9 to bind to the T7 promoter and inhibit the function of the promoter.
How does it work?
Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination has the effect of inhibition of targeted gene transcription. This process can silence gene expression in cells effectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]