Difference between revisions of "Part:BBa K3777023"

Revision as of 17:28, 21 October 2021

T7-3WJdB

Basic biosensor device for tetracycline detection.

Usage and Biology
The curcuit includes 3WJdB(BBa_K3777000) which is under the control of T7 promoter (BBa_K3777006). The terminator we used was BBa_M36305.(Fig 1)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 206
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 <!-- Add more about the biology of this part here-->
 <b><font size="3">Usage and Biology</font></b>
-<br>3WJdB(BBa_K3777000) under the control of T7 promoter (BBa_K3777006).  The terminator we used were BBa_B0010 and BBa_M36305.(Fig 1)
+<br>The curcuit includes 3WJdB(BBa_K3777000) which is under the control of T7 promoter (BBa_K3777006).  The terminator we used was BBa_M36305.(Fig 1)
-<br>When tet was absent, TetR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene.
+https://static.igem.org/mediawiki/parts/thumb/8/88/T7-3WJdB.PNG/800px-T7-3WJdB.PNG
-<br>We expressed this circuit in the <i>E. coli </i> BL21(DE3) cells for tetracycline detection. Thus we could roughly deduce the concentration of the antibiotics in the sample according to the fluorescence intensity.
-https://static.igem.org/mediawiki/parts/thumb/3/3d/Tetr-tetO-3WJdB.PNG/799px-Tetr-tetO-3WJdB.PNG
+<br>
-<br>                           Fig.1  Schematic overview of the genetic circuit.
-<br><b><font size="3">Results</font></b>
-<br>To verify the functionality of the biosensor, we performed a plate-reader experiment and measured optical density and fluorescence intensity every hour. We observed a correlation between concentration of antibiotics in the sample and intensity of fluorescent signal.
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