Revision as of 17:10, 21 October 2021

Sec system (PhoA), TEV protease with universal expression

This composite is using a constitutive family promoter and artificial terminator to enable expression under non T7 vectors. This composite is one of two that will be working to generate a TEV protease. This composite is expressed extracellularly through the Sec pathway. Characterization of two expression systems (between universal and T7) is measured to ensure great efficiency of expression. Characterization of the overall system contributes to improving the new era of protein overexpression.

Introduction

UBrawijaya iGEM 2021 project CHOP systemis an outer membrane vesicle based protein overexpression system that includes three goals towards producing a blueprint for an ideal protein production system. This project can be customized because the system can be adapted with various proteins of interest, the overexpression system that can increase protein yield, and the clean harvest with simplified purification steps that is equipped with a protease enzyme expression mechanism in the concentrated system - from vesicles and cell pellets.

This composite aims to express TEV protease through constitutive promoter and translocates TEV protease across the cell membrane and out of the cell through the Sec pathway under PhoA signal sequence.

Result

Sequence and Features