Difference between revisions of "Part:BBa K3905000"

Latest revision as of 16:22, 21 October 2021

gen1 517-5p Toehold Switch

Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 15
Illegal XbaI site found at 53
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 15
Illegal XbaI site found at 53
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 15
Illegal XbaI site found at 53
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3905000 short</partinfo>
-Toehold switch that regulates the translation of Luciferase. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms duplex. This duplex trigger causes the toehold switch to unfold by binding to the toehold sequence, exposing the RBS and start codon to allow the translation of luciferase. Luciferase production can be measured by adding luciferin and using a luminometer to measure the intensity of the light.
+Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
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