Difference between revisions of "Part:BBa K4032105"
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Contents: | Contents: | ||
− | ・The lac promoter | + | ・The lac promoter from <partinfo>BBa_R0010</partinfo> |
− | ・The RBS | + | ・The RBS from <partinfo>BBa_B0034</partinfo> |
− | ・The gene of DegP protein | + | ・The gene of DegP protein from <partinfo>BBa_K4032002</partinfo> |
− | ・The double terminator | + | ・The double terminator from <partinfo>BBa_B0015</partinfo> |
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− | === | + | ===Design=== |
The lac promoter, the RBS and the double terminator are added to <partinfo>BBa_K4032002</partinfo>, forming this part. | The lac promoter, the RBS and the double terminator are added to <partinfo>BBa_K4032002</partinfo>, forming this part. |
Latest revision as of 16:07, 21 October 2021
lacI+RBS+DegP+double terminator
Contents:
・The lac promoter from BBa_R0010
・The RBS from BBa_B0034
・The gene of DegP protein from BBa_K4032002
・The double terminator from BBa_B0015
Usage and Biology
DegP is a type of heat shock protein in E. coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.
It is also a type of serine protease in terms of the presence of serine residues in the active center.
In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.
Design
The lac promoter, the RBS and the double terminator are added to BBa_K4032002, forming this part.
The lac promoter, the RBS and the double terminator are from BBa_J04450.
This part was created by the In-Fusion method.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1364
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1364
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1364
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1364
Illegal AgeI site found at 2206
Illegal AgeI site found at 2318 - 1000COMPATIBLE WITH RFC[1000]