Difference between revisions of "Part:BBa K3788011"

m
 
Line 3: Line 3:
  
  
−
BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, the mutation of the 846 – 847 amino acid permit to have no protein cleavage, and keep it attached at the extracellular membrane.
+
<p>BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.</p>
 +
 
 +
<p>The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.</p>
 +
 
 +
<p>Wild-Type Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium, but we introduced D:33N substitution the the cleavage site is not recognized anymore and the passenger protein remains attached to the cell.</p>
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:52, 21 October 2021

NOTOC Aida-I (D33:N) T5SS


BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.

The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.

Wild-Type Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium, but we introduced D:33N substitution the the cleavage site is not recognized anymore and the passenger protein remains attached to the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1084
  • 1000
    COMPATIBLE WITH RFC[1000]