Difference between revisions of "Part:BBa K3788011"
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+ | NOTOC | ||
+ | <partinfo>BBa_K3788011 short</partinfo> | ||
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+ | <p>BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.</p> | ||
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+ | <p>The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.</p> | ||
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+ | <p>Wild-Type Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium, but we introduced D:33N substitution the the cleavage site is not recognized anymore and the passenger protein remains attached to the cell.</p> | ||
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+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
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+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3788011 SequenceAndFeatures</partinfo> | ||
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+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3788011 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 15:52, 21 October 2021
NOTOC Aida-I (D33:N) T5SS
BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.
The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.
Wild-Type Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium, but we introduced D:33N substitution the the cleavage site is not recognized anymore and the passenger protein remains attached to the cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1084
- 1000COMPATIBLE WITH RFC[1000]