Difference between revisions of "Part:BBa K3788010"

Latest revision as of 15:45, 21 October 2021

Aida-I (T5SS)

Part: BBa K3788010 is coding Aida-I, it is an autotransporter from the T5SS; it allow the addressing to the extracellular medium.

The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.

Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1084
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3788010 short</partinfo>
-Part: BBa K3788010 is coding for the sfGFP by without STOP. The sequence Part:BBa_I746916 where the 2 stop codon "tgatga" were delated.
+<p>Part: BBa K3788010 is coding Aida-I, it is an autotransporter from the T5SS; it allow the addressing to the extracellular medium.</p>
+<p>The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.</p>
+<p>Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium.</p>
 <!-- Add more about the biology of this part here