Difference between revisions of "Part:BBa K3788010"

 
 
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<partinfo>BBa_K3788010 short</partinfo>
 
<partinfo>BBa_K3788010 short</partinfo>
  
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BBa_K3788010 is coding for pelB, sfGFP and AidaA. pelB is a leader sequence, it allows the protein to join the periplasm via the SEC system (unfolded proteins). Aida-I, on the other hand, is an autotransporter from the T5SS; it allow the addressing to the extracellular medium. In this contruction sfGFP permit to understand the mechanism, this protein can at the end be replaced by an other protein.
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<p>Part: BBa K3788010 is coding Aida-I, it is an autotransporter from the T5SS; it allow the addressing to the extracellular medium.</p>
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sfGFP is supposed to be address to the preriplasm thanks to pelB leader and the T5SS system will address sfGFP to the extracellular medium by a cleavage.  
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Sequence was optimised for E. coli K12.
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<p>The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.</p>
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<p>Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:45, 21 October 2021


Aida-I (T5SS)

Part: BBa K3788010 is coding Aida-I, it is an autotransporter from the T5SS; it allow the addressing to the extracellular medium.

The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.

Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1084
  • 1000
    COMPATIBLE WITH RFC[1000]