Difference between revisions of "Part:BBa K4080008:Design"
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | no | + | <ul> |
− | + | <li> | |
− | + | Besides the burden value of each part and basic criteria like illegal restriction sites and any other stuff that could burden the assembly, there is no specific consideration to deal with | |
+ | </li> | ||
+ | <li> | ||
+ | The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX | ||
+ | </li> | ||
+ | <li> | ||
+ | Linkers placed between parts : GGG or GSG | ||
+ | </li> | ||
+ | </ul> | ||
===Source=== | ===Source=== | ||
− | + | All of the parts are from a previous iGEM team that registered in the parts registry | |
===References=== | ===References=== |
Revision as of 15:42, 21 October 2021
TEV protease-eCPX fusion with universal expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Besides the burden value of each part and basic criteria like illegal restriction sites and any other stuff that could burden the assembly, there is no specific consideration to deal with
- The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
- Linkers placed between parts : GGG or GSG
Source
All of the parts are from a previous iGEM team that registered in the parts registry