Difference between revisions of "Part:BBa K3788013:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| − | <p>We can see that bacteria containing J04500 do not emit light, contrary to the ones containing | + | <p>We can see that bacteria containing J04500 do not emit light, contrary to the ones containing BBa_K3788013, which are green under a confocal microscope.</p> |
<p>However, we are not able to determine whether the light is located at the surface of the bacteria or if it is located in all the cells.</p> | <p>However, we are not able to determine whether the light is located at the surface of the bacteria or if it is located in all the cells.</p> | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:38, 21 October 2021
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how you used this part and how it worked out.
Applications of BBa_K3788013
Cell visualization with confocal microscope
We wanted to determine whether the fluorescence is located in the periplasm or in the cytoplasm, so we used a confocal microscope to visualize our cells.
We transformed E.Coli MG1655 strains with an empty J04500 vector or with the BBa_K3788013 plasmid. We then prepared 3mL 2YT growing medium with chloramphenicol and then put MG1655 containing either J04500 or BBa_K3788025 plasmids.
After 3 hours of incubation at 37°C, we prepared the plates and visualized our cells
Figure : Visualisation of MG1655 E.coli strains containing J04500
Figure : Visualisation of MG1655 E.coli strains containing BBa_K3788013
We can see that bacteria containing J04500 do not emit light, contrary to the ones containing BBa_K3788013, which are green under a confocal microscope.
However, we are not able to determine whether the light is located at the surface of the bacteria or if it is located in all the cells.
User Reviews
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