Difference between revisions of "Part:BBa K3861025"
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| + | Composite part consisting of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861000">BBa_K3861000</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861012">BBa_K3861012</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS. The HiBit tagged Pep8 allows to track the Pep8 in translocation assays. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 15:33, 21 October 2021
PlldR-sicP-sptP-Pep8-HiBiT
Composite part consisting of BBa_K3861000 and BBa_K3861012. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS. The HiBit tagged Pep8 allows to track the Pep8 in translocation assays.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 519
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal PstI site found at 519 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 519
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 519
- 1000COMPATIBLE WITH RFC[1000]