Difference between revisions of "Part:BBa K3861024"
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| + | Composite part consisting of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861000">BBa_K3861000</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861010">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI-1 T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 15:33, 21 October 2021
PlldR-sicP-sptP-Pep8
Composite part consisting of BBa_K3861000 and BBa_K3861010. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI-1 T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 519
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal PstI site found at 519 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 519
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 519
- 1000COMPATIBLE WITH RFC[1000]