Difference between revisions of "Part:BBa K3982002"

Latest revision as of 15:04, 21 October 2021

Tobacco Etch Virus (TEV) protease

Tobacco Etch Virus (TEV) protease is used in Project CODE M by Team IISER_Berhampur 2021

Usage and Biology

TEV protease is a highly specific protease from Tobacco Etch Virus. It is used for controlled cleavage of fusion proteins and thus, their easy purification in in-vitro and in-vivo experiments. The cleavage site is between Gln and Gly/Ser residues.

Reference

Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science (New York, N.Y.), 362(6416), 839–842. https://doi.org/10.1126/science.aav4294

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Tobacco Etch Virus (TEV) protease is used in Project CODE M by [https://2021.igem.org/Team:IISER_Berhampur/ Team IISER_Berhampur 2021]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 TEV protease is a highly specific protease from Tobacco Etch Virus. It is used for controlled cleavage of fusion proteins and thus, their easy purification in in-vitro and in-vivo experiments. The cleavage site is between Gln and Gly/Ser residues.
 ===Reference===
-L. B. Harrington et al., Science, 10.1126/science.aav4294 (2018).
+Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science (New York, N.Y.), 362(6416), 839–842. https://doi.org/10.1126/science.aav4294
 <!-- -->