Difference between revisions of "Part:BBa K3764001"

 
 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3764001 short</partinfo>
 
<partinfo>BBa_K3764001 short</partinfo>
 +
  
 
This part is a modified version of part BBa_K3764000. It contains the same specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion but here we integrated it into the ORF of an mRFP1 protein instead of using LacZalfa fragment. With this version, one can use the color-based selection option without adding IPTG, X-Gal and/or using specialized E. coli strains for cloning.
 
This part is a modified version of part BBa_K3764000. It contains the same specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion but here we integrated it into the ORF of an mRFP1 protein instead of using LacZalfa fragment. With this version, one can use the color-based selection option without adding IPTG, X-Gal and/or using specialized E. coli strains for cloning.
 +
  
 
Once integrated into a BioBrick vector it will transform the plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
 
Once integrated into a BioBrick vector it will transform the plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
 +
  
 
This part has no promoter and RBS module! You should add one if you like to use the color-based selection.
 
This part has no promoter and RBS module! You should add one if you like to use the color-based selection.
 +
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:57, 21 October 2021


Module for generation of a T-vector with mRFP1 based selection


This part is a modified version of part BBa_K3764000. It contains the same specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion but here we integrated it into the ORF of an mRFP1 protein instead of using LacZalfa fragment. With this version, one can use the color-based selection option without adding IPTG, X-Gal and/or using specialized E. coli strains for cloning.


Once integrated into a BioBrick vector it will transform the plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.


This part has no promoter and RBS module! You should add one if you like to use the color-based selection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 43
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 51
    Illegal BamHI site found at 160
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 750
    Illegal AgeI site found at 862
  • 1000
    COMPATIBLE WITH RFC[1000]