Difference between revisions of "Part:BBa K3814017"

 
 
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<partinfo>BBa_K3814017 short</partinfo>
 
<partinfo>BBa_K3814017 short</partinfo>
  
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This 50bp section of DNA is going to be used as a homology arm to insert the next gene cluster into. These bases have been designed to minimise self-dimerisation and hairpin formations, as it will also exist as a separate primer.  
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This 50bp section of DNA is random DNA that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome. This and all other homology arms listed in the parts registry can be used by future teams. This homology arm have been designed to minimise self-dimerisation and hairpin formations, as in USYD 2021's project, it will also exist as a separate primer.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:54, 21 October 2021


5' Homology Arm for Cluster 4

This 50bp section of DNA is random DNA that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome. This and all other homology arms listed in the parts registry can be used by future teams. This homology arm have been designed to minimise self-dimerisation and hairpin formations, as in USYD 2021's project, it will also exist as a separate primer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]