Difference between revisions of "Part:BBa K3788017:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | <h1>Applications of BBa_K3788017</h1> |
| + | ===Methods=== | ||
| + | <p>In order to characterize the promoter strength, we fused it to the GFP encoding sequence BBa_E0040 and to the timer-lysis device part <b>BBa_K3788018</b> (composite part <b>BBa_K3788019</b>). Induction of the SOS response was induced by mitomycin C treatments and the fluorescence was recorded in a dose-dependent manner using a microplate reader. Following iGEM Measurement recommendations, the GFP fluorescence intensity was converted to a concentration of fluorescein protein thanks to a standard curve of fluorescein dilution series.</p> | ||
| + | <br> | ||
| + | ===Results=== | ||
| + | <p> The cells carrying the <b>BBa_K3788019</b> construct were grown with various concentrations of mitomycin, ranging from 0 to 800 ng.ml-1. The OD (600 nm) and the amount of green fluorescence were recorded over time.</p> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:32, 21 October 2021
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3788017
Methods
In order to characterize the promoter strength, we fused it to the GFP encoding sequence BBa_E0040 and to the timer-lysis device part BBa_K3788018 (composite part BBa_K3788019). Induction of the SOS response was induced by mitomycin C treatments and the fluorescence was recorded in a dose-dependent manner using a microplate reader. Following iGEM Measurement recommendations, the GFP fluorescence intensity was converted to a concentration of fluorescein protein thanks to a standard curve of fluorescein dilution series.
Results
The cells carrying the BBa_K3788019 construct were grown with various concentrations of mitomycin, ranging from 0 to 800 ng.ml-1. The OD (600 nm) and the amount of green fluorescence were recorded over time.
User Reviews
UNIQ36d9d37ba3dbe159-partinfo-00000000-QINU UNIQ36d9d37ba3dbe159-partinfo-00000001-QINU