Difference between revisions of "Part:BBa K3776011"
(One intermediate revision by the same user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K3776011 short</partinfo> | <partinfo>BBa_K3776011 short</partinfo> | ||
− | This | + | This is Neutral Site I (NSII) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010). |
+ | Our team is creating a crRNA targeting NSII for CRISPR technology to edit the genome of S.elongatus 2973. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 14:19, 21 October 2021
NSII
This is Neutral Site I (NSII) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010). Our team is creating a crRNA targeting NSII for CRISPR technology to edit the genome of S.elongatus 2973.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1119
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]