Difference between revisions of "Part:BBa K3814002"

 
 
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<partinfo>BBa_K3814002 short</partinfo>
 
<partinfo>BBa_K3814002 short</partinfo>
  
Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced. The modification is described below and came from the following paper: https://doi.org/10.1038/s41589-018-0168-3
+
Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced.  
  
Promoter   -35     -10
+
{|
Psal         TTATCA   TATCGT
+
|'''Promoter'''
PsalTTC   TTATTT   TACCGT
+
|'''-35'''
 +
|'''-10'''
 +
|-
 +
|Psal
 +
|TTATCA
 +
|TATCGT
 +
|-
 +
|PsalTTC
 +
|TTATTT
 +
|TACCGT
 +
|}
  
 +
 +
This example showcases the WT PsalTTC variant. This is described more indepth in the design section.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:03, 21 October 2021


Psal1 (PsalTTC, 100% Activity)

Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced.

Promoter -35 -10
Psal TTATCA TATCGT
PsalTTC TTATTT TACCGT


This example showcases the WT PsalTTC variant. This is described more indepth in the design section.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 68
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]