Difference between revisions of "Part:BBa K3921008"

 
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<partinfo>BBa_K3921008 short</partinfo>
 
<partinfo>BBa_K3921008 short</partinfo>
  
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RNAi system containing PcpccG2-172 promoter.Under the green light of 520nm, CcaS (green circle) is self-phosphorylated, then the phosphate group (yellow circle) is transferred to the corresponding regulatory factor CcaR (red circle), and the phosphorylated CcaR binds to the cpcG2 promoter to start the expression of DicF. The process is reversed at 660nm red light.
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RNAi system containing PcpccG2-172 promoter.Under the green light of 520nm, CcaS (green circle) is self-phosphorylated, then the phosphate group (yellow circle) is transferred to the corresponding regulatory factor CcaR (red circle), and the phosphorylated CcaR binds to the cpcG2 promoter to start the expression of DicF and cell division is inhibited. when induced by red light of 660nm, DicF is not expressed and cells divide normally.
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<p class="MsoNormal">https://2021.igem.org/wiki/images/2/24/T--XHD-Wuhan-B-China--Proof7.png</p>
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<p class="MsoNormal"><strong>Figure 1. Schematics of the 172_dicF_ccaR&S circuit..</strong></p>
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<p class="MsoNormal">&nbsp;</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:44, 21 October 2021


172-dicF-ccaR&ccaS

RNAi system containing PcpccG2-172 promoter.Under the green light of 520nm, CcaS (green circle) is self-phosphorylated, then the phosphate group (yellow circle) is transferred to the corresponding regulatory factor CcaR (red circle), and the phosphorylated CcaR binds to the cpcG2 promoter to start the expression of DicF and cell division is inhibited. when induced by red light of 660nm, DicF is not expressed and cells divide normally.

T--XHD-Wuhan-B-China--Proof7.png

Figure 1. Schematics of the 172_dicF_ccaR&S circuit..

 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 445
    Illegal NheI site found at 468
    Illegal NheI site found at 1359
    Illegal NheI site found at 1382
    Illegal NheI site found at 2042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 901
    Illegal XhoI site found at 3688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2706
  • 1000
    COMPATIBLE WITH RFC[1000]