Difference between revisions of "Part:BBa K239000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI.  
 
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI.  
−
Only SpeI restriction site had to be removed.
+
Only one SpeI restriction site had to be removed.
 
Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.
 
Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.
  

Revision as of 09:40, 21 June 2009

DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. Only one SpeI restriction site had to be removed. Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References