Difference between revisions of "Part:BBa K239000:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K239000 short</partinfo>
 
<partinfo>BBa_K239000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Detect and remove restriction sites: Xba1, EcoRI, Pst1, Spe1.
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Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI.
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Some uncertanty about where to start and stop in the sequece. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.  
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Only SpeI restriction site had to be removed.
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+
Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.
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===Source===
 
===Source===

Revision as of 09:40, 21 June 2009

DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. Only SpeI restriction site had to be removed. Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References