Difference between revisions of "Part:BBa K3714004"
Ricardo Su (Talk | contribs) |
|||
| Line 11: | Line 11: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3714004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3714004 SequenceAndFeatures</partinfo> | ||
| − | < | + | <hr/> |
| − | By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1). | + | By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1). Our aptazyme exhibits a 2-fold change of cleavage proportion under 10μM at ~1mg/ml (0.125x dilution of saturated solution at room temperature) while the origin apatazyme (BBa_J119449) shows only 1.3-fold change. |
<center>[[File:K3714004.png]]</center> | <center>[[File:K3714004.png]]</center> | ||
Revision as of 12:48, 21 October 2021
Improved theophylline biosensor
An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1). Our aptazyme exhibits a 2-fold change of cleavage proportion under 10μM at ~1mg/ml (0.125x dilution of saturated solution at room temperature) while the origin apatazyme (BBa_J119449) shows only 1.3-fold change.
