Difference between revisions of "Part:BBa K4011007"
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Revision as of 12:39, 21 October 2021


SaACS2

SaACS2 is an acetyl-CoA synthase, responsible for synthesizing acetyl-CoA from a host of different substrates. This year, we will express SaACS2 in yeast cells to turn acetate into acetyl-CoA, providing the first step for our metabolic pathway which converts acetate and ethanol into ethyl-acetate. The part will be used to construct yeast expression plasmids through golden gate assembly into pTEF-SaACS2-tADH1BBa_K4011016 and pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 BBa_K4011019.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1537
  • 1000
    COMPATIBLE WITH RFC[1000]