Difference between revisions of "Part:BBa K4011012"
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Revision as of 12:14, 21 October 2021


pTac-RiboJ-Fre-SttH-B0015

The DNA circuit of ptac-RiboJ-Fre-SttH-B0015 is constructed from an IPTG inducible promoter of E.coli: ptac-RiboJ, a fusion protein which can express halogenase: Fre-SttH, and a strong terminator: B0015. This complete DNA circuit can achieve the halogenation of 6th carbon of tryptophan in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1928
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1776