Difference between revisions of "Part:BBa K3999002"

(→‎Properties)
(→‎Properties: a plasmid backbone)
 
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====Origin: Synthetic====
 
====Origin: Synthetic====
 
====Properties: a plasmid backbone====
 
====Properties: a plasmid backbone====
 +
==Usage and biology==
 
This sequence contains multiple cloning sites (MCS), used for restriction digest assembly of our pTRC99K plasmids. It also contains the promoter, coding sequence, and terminator for streptomyces kanamyceticus resistance (KanR). It contains an origin of replication in E.coli for the plasmid, a LacI promoter, a LacI coding sequence for a lac repressor, a Lac operator, and a trp promoter within lacUV5.
 
This sequence contains multiple cloning sites (MCS), used for restriction digest assembly of our pTRC99K plasmids. It also contains the promoter, coding sequence, and terminator for streptomyces kanamyceticus resistance (KanR). It contains an origin of replication in E.coli for the plasmid, a LacI promoter, a LacI coding sequence for a lac repressor, a Lac operator, and a trp promoter within lacUV5.
 
[[File:T--Shanghai Metro--Figure1.Plasmid map of pTRC99K.png|500px|thumb|center|Figure1. Plasmid map of pTRC99K.]]
 
[[File:T--Shanghai Metro--Figure1.Plasmid map of pTRC99K.png|500px|thumb|center|Figure1. Plasmid map of pTRC99K.]]

Latest revision as of 11:21, 21 October 2021


pTrc99k

pTrc99k

Profile

Name: pTrc99k

Base Pairs:4167bp

Origin: Synthetic

Properties: a plasmid backbone

Usage and biology

This sequence contains multiple cloning sites (MCS), used for restriction digest assembly of our pTRC99K plasmids. It also contains the promoter, coding sequence, and terminator for streptomyces kanamyceticus resistance (KanR). It contains an origin of replication in E.coli for the plasmid, a LacI promoter, a LacI coding sequence for a lac repressor, a Lac operator, and a trp promoter within lacUV5.

Figure1. Plasmid map of pTRC99K.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1
    Illegal SapI.rc site found at 3967