Difference between revisions of "Part:BBa K3720003"

 
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When used in combination with CooA, it is possible to create a light emitting mechanism that reacts with carbon monoxide.
 
When used in combination with CooA, it is possible to create a light emitting mechanism that reacts with carbon monoxide.
 +
Culture pBEST incorporating this part in LD medium and transfer 20㎕ to a 1.5-ml tube.
 +
Then, 0.5㎕ of D-luciferin was added, CO was injected, and luminescence was measured.
 +
Quantitative measurements were not possible because the laboratory facilities did not allow us to prepare a luminometer.
 +
As shown in the photo, the pipette which carbon monoxide was injected showed a change in color.
 +
Left: LB medium+luciferin
 +
Right: LB medium+luciferin+CO gas
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:50, 21 October 2021


Firefly luciferase with pCooM, RBS (weak), and double terminator

When used in combination with CooA, it is possible to create a light emitting mechanism that reacts with carbon monoxide. Culture pBEST incorporating this part in LD medium and transfer 20㎕ to a 1.5-ml tube. Then, 0.5㎕ of D-luciferin was added, CO was injected, and luminescence was measured. Quantitative measurements were not possible because the laboratory facilities did not allow us to prepare a luminometer. As shown in the photo, the pipette which carbon monoxide was injected showed a change in color. Left: LB medium+luciferin Right: LB medium+luciferin+CO gas

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 900