Difference between revisions of "Part:BBa K4023001"

Revision as of 10:34, 21 October 2021

Optimized glpF

glpF codes for a 28kDa¹ transmembrane glycerol uptake facilitator protein found in E.coli strain. glpF protein catalyze transmembrane diffusion of glycerol and some linear polyhydric alcohols in E.coli¹. glpF protein also allows Arsenite to be transported into E.coli. This is as Arsenite is protonated at physiological pH and forms As(OH)3 as a major species. As(OH)3 is chemically analogous to glycerol².

References

1. Fu, D., Libson, A., & Stroud, R. (2002, April). The structure of G1pF, a glycerol conducting channel. In Ion Channels: From Atomic Resolution Physiology to Functional Genomics: Novartis Foundation Symposium 245 (Vol. 245, pp. 51-65). Chichester, UK: John Wiley & Sons, Ltd.

2. Bhattacharjee, H., Mukhopadhyay, R., Thiyagarajan, S., & Rosen, B. P. (2008). Aquaglyceroporins: ancient channels for metalloids. Journal of biology, 7(9), 1-6.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 183

@@ Line 7: / Line 7: @@
 ===References===
 . Fu, D., Libson, A., & Stroud, R. (2002, April). The structure of G1pF, a glycerol conducting channel. In Ion Channels: From Atomic Resolution Physiology to Functional Genomics: Novartis Foundation Symposium 245 (Vol. 245, pp. 51-65). Chichester, UK: John Wiley & Sons, Ltd.
 . Bhattacharjee, H., Mukhopadhyay, R., Thiyagarajan, S., & Rosen, B. P. (2008). Aquaglyceroporins: ancient channels for metalloids. Journal of biology, 7(9), 1-6.