Difference between revisions of "Part:BBa K4012001"
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===Obtaining the pTEF1 fragment and BsaI digested verification===
 
===Obtaining the pTEF1 fragment and BsaI digested verification===
  
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[[Image:T--AISSU_Union--partslast.jpg|thumbnail|500px|center|'''Figure 1:'''  
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[[Image:T--AISSU_Union--partslast.jpg|thumbnail|1000px|center|'''Figure 1:'''  
 
[https://parts.igem.org/Part:BBa_K4012001] Results of yeast toolkit plasmids enzyme-digested verification]]
 
[https://parts.igem.org/Part:BBa_K4012001] Results of yeast toolkit plasmids enzyme-digested verification]]
 
We construct pTEF1 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments pTEF1(709bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.
 
We construct pTEF1 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments pTEF1(709bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.
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===pTEF1 in Level1 plasmid assembly===
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[[Image:T--AISSU_Union--partslast.jpg|thumbnail|1000px|center|'''Figure 1:'''
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[https://parts.igem.org/Part:BBa_K4012001] Results of yeast toolkit plasmids enzyme-digested verification]]

Revision as of 10:21, 21 October 2021


pTEF1

A promoter sequence in genome of the Saccharomyces cerevisiae genome, which is used to activate the CHS sequence from Ginkgo biloba in our reconbined plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 724


Obtaining the pTEF1 fragment and BsaI digested verification

Figure 1: [1] Results of yeast toolkit plasmids enzyme-digested verification

We construct pTEF1 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments pTEF1(709bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.

pTEF1 in Level1 plasmid assembly

Figure 1: [2] Results of yeast toolkit plasmids enzyme-digested verification