Difference between revisions of "Part:BBa K3776006"
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<partinfo>BBa_K3776006 short</partinfo>
 
<partinfo>BBa_K3776006 short</partinfo>
  
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This part allows overproduction of lysine by a feedback insensitive AK and conversion of L-lysine to 5-aminovaleramide (5AVA) by DavB and DavA in Synechococcus elongatus UTEX 2973. Promoters Pcpc560, 6803PsbA2 and terminator TrbcL are from the genome Synechocystis sp. PCC6803.
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This part allows overproduction of L-lysine by a feedback insensitive AK and conversion of L-lysine to 5-aminovaleramide (5-AVA) by DavB and DavA in Synechococcus elongatus UTEX 2973. Promoters Pcpc560, 6803PsbA2 and terminator TrbcL are from the genome Synechocystis sp. PCC6803.
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In the L-lysine biosynthetic pathway in cyanobacteria, aspartate kinase (AK) catalyzes the conversion of L-aspartate to L- aspartyl-phosphate and is subject to negative feedback by L-lysine, having decreased activity when there are high levels of L-lysine.
  
 
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Revision as of 10:10, 21 October 2021


davB-davA-AK

This part allows overproduction of L-lysine by a feedback insensitive AK and conversion of L-lysine to 5-aminovaleramide (5-AVA) by DavB and DavA in Synechococcus elongatus UTEX 2973. Promoters Pcpc560, 6803PsbA2 and terminator TrbcL are from the genome Synechocystis sp. PCC6803.

In the L-lysine biosynthetic pathway in cyanobacteria, aspartate kinase (AK) catalyzes the conversion of L-aspartate to L- aspartyl-phosphate and is subject to negative feedback by L-lysine, having decreased activity when there are high levels of L-lysine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 800
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1889
    Illegal BglII site found at 4844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2049
    Illegal NgoMIV site found at 3816
    Illegal AgeI site found at 1456
    Illegal AgeI site found at 1705
    Illegal AgeI site found at 3040
    Illegal AgeI site found at 5043
  • 1000
    COMPATIBLE WITH RFC[1000]