Difference between revisions of "Part:BBa K4012010"
(→‎Obtaining the pTEF1 fragment and BsaI digested verification)
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===Obtaining the pTEF1 fragment and BsaI digested verification===
 
===Obtaining the pTEF1 fragment and BsaI digested verification===
  
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[[Image:https://2021.igem.org/wiki/images/0/09/T--AISSU_Union--partslast.jpg|thumbnail|500px|center|'''Figure.1:'''  
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[[Image:T--AISSU_Union--partslast.jpg|thumbnail|500px|center|'''Figure.1:'''  
 
[https://parts.igem.org/Part:BBa_K4012001] Results of yeast toolkit plasmids enzyme-digested verification]]
 
[https://parts.igem.org/Part:BBa_K4012001] Results of yeast toolkit plasmids enzyme-digested verification]]
  
 
           <p>Fig.1 Results of yeast toolkit plasmids enzyme-digested verification</p>
 
           <p>Fig.1 Results of yeast toolkit plasmids enzyme-digested verification</p>

Revision as of 10:08, 21 October 2021


GbCHS

GbCHS is a sequence code for Chalcone synthase in Anthurium andraeanum. The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. It is a Acyltransferase involved in Flavonoid biosynthesis. The catalytic activity: 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 891
    Illegal SapI.rc site found at 840



Obtaining the pTEF1 fragment and BsaI digested verification

Figure.1: [1] Results of yeast toolkit plasmids enzyme-digested verification

Fig.1 Results of yeast toolkit plasmids enzyme-digested verification