Difference between revisions of "Part:BBa K4012010"
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===Obtaining the pTEF1 fragment and BsaI digested verification===
 
===Obtaining the pTEF1 fragment and BsaI digested verification===
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<img src=https://2021.igem.org/wiki/images/8/83/T--AISSU_Union--Fig.1_Results_of_yeast_toolkit_plasmids_enzyme-digested_verification.jpg>
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          <p>Fig.1 Results of yeast toolkit plasmids enzyme-digested verification</p>

Revision as of 09:46, 21 October 2021


GbCHS

GbCHS is a sequence code for Chalcone synthase in Anthurium andraeanum. The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. It is a Acyltransferase involved in Flavonoid biosynthesis. The catalytic activity: 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 891
    Illegal SapI.rc site found at 840



Obtaining the pTEF1 fragment and BsaI digested verification

<img src=T--AISSU_Union--Fig.1_Results_of_yeast_toolkit_plasmids_enzyme-digested_verification.jpg>

Fig.1 Results of yeast toolkit plasmids enzyme-digested verification